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. 2011 Oct 3;14(1):19–28. doi: 10.1093/neuonc/nor168

Fig. 2.

Fig. 2.

Effects of DHMEQ on cellular growth and nuclear translocation of RelA in glioblastoma cell lines. (A) Effects of varying concentrations of DHMEQ (0–100 µg/mL) on the growth of U87, YKG-1, U251, A172, and T98G. Cells were cultured in growth medium (DMEM, 10% FBS) for indicated periods. Error bars, SD (n = 3). (B) Effects of DHMEQ (10 µg/mL) on TNFα-induced (20 ng/mL) nuclear translocation of RelA in U87 (upper panel) and uPA expression in U251 cells (lower panel). For RelA translocation, immunofluorescence study (green, RelA; blue, nuclei) and immunoblot analysis of nuclear extracts of U87 cells are shown. Nuclear translocation of RelA after 30 min of TNFα stimulation was disturbed by DHMEQ pretreatment (2.5 h). RT-PCR analysis for the expression of uPA indicated that DHMEQ pretreatment (6 h) suppressed the TNFα-induced uPA upregulation.