Fig. 3.
Effects of DHMEQ on cell cycle and apoptosis in glioblastoma cell lines. (A) Representative DNA cell cycle histograms of vehicle (DMSO)–treated and DHMEQ-treated (10 µg/mL) U87 (48-h treatment) and YKG-1 (12-h treatment) cells. (B) Time course of G2/M arrest. Ratios of the proportion of G2/M subpopulation in DHMEQ-treated cells to that of vehicle-treated cells are plotted (mean ± SD of 5 independent experiments). Mean values of G2/M proportion (%) in the nontreated cells and that in the cells showing peak effect are also indicated. *P < .001, compared with control (Mann-Whitney U test).