Fig. 6.
Effect of HgCl2 and CH3HgCl on the rhGTK-catalyzed transamination of L-phenylalanine with KMB. The reaction mixture (50 μl) contained 20 mM L-phenylalanine, 5 mM KMB, 2.6 mU of enzyme, 100 mM potassium phosphate buffer (pH 7.4) in the presence or absence of 1 mM HgCl2 or CH3HgCl. Samples were incubated at 37°C and phenylpyruvate formation was measured at the times shown (n = 3).