. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: Arch Biochem Biophys. 2011 Nov 10;517(1):20–29. doi: 10.1016/j.abb.2011.11.002

Table 1.

Inhibition of rhGTK-catalyzed transamination of L-phenylalanine with KMB by Cys- and Hcy-S-conjugates of Hg²⁺ and CH₃Hg-

Inhibitor	L-Phenylalanine concentration
	10 mM	20 mM	50 mM

	Relative phenylpyruvate formation (%)
None	[100]	[100]	[100]

CH₃Hg-S-Cys (0.5 mM)	72 ± 26	55 ± 11	63 ± 25
CH₃Hg-S-Hcy (0.5 mM)	49 ± 11	40 ± 4	50 ± 3
Cys-S-Hg-S-Cys (1 mM)	31 ± 4	29 ± 8	33 ± 6
Hcy-S-Hg-S-Hcy (0.5 mM)	43 ±5	35 ± 4	50 ± 7

The reaction mixture contained varying concentrations of L-phenylalanine, 5 mM KMB, 100 mM potassium phosphate buffer (pH 7.4) and enzyme (2 mU) in a final volume of 50 μl in the presence or absence of mercury S-conjugate. After incubation for 30 min at 37 °C, phenylpyruvate formation was determined. N = 4 or 5.