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. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: Immunity. 2011 Dec 15;35(6):972–985. doi: 10.1016/j.immuni.2011.09.019

Figure 6. IL-17A-secreting cells maintain the ability to self-renew upon transfer into a highly activating environment and contribute to the effective rejection of tumor.

Figure 6

A total of 1×106 in vitro polarized Th17 and Th1 TRP-1 cells (Thy1.2+) were adoptively transferred into sublethally irradiated (5Gy) B6.PL (Thy1.1+) mice. On day 36 following transfer spleens from recipient mice were harvested and stimulated for 6 hours with TRP-1 peptide in presence of brefeldin A.

(A) Representative dot plots show intracellular IFN-γ and IL-17A in Thy1.2+ cells in the indicated experimental groups.

(B) Bar graphs show mean frequency of IFN-γ- and IL-17A-secreting cells among surviving Thy1.2+ Th1- and Th17-derived cells 36 days after adoptive transfer.

(C) Purified CD4+ cells from C57/B6 and IL-17A−/− donors were stimulated under Th17 conditions and transduced with retroviral vector encoding for TRP-1 TCR. A total of 1.2×106 cells were adoptively transferred into 5Gy irradiated C57BL/6 mice. Tumor growth was measured serially. Error bars represent SEM (n=5–6); (*=p<0.05, **=p<0.01, ***=p<0.001).