Table 2.
Zeta Potential measurement of reference and 3 years samples
| Years in storage1 | Sample Form | Sample ID | Zeta Potential (mV) |
|---|---|---|---|
| 0 (Reference2) | Aqueous Solution | AMA1-C1 Antigen in saline | -23.22 ± 1.48 |
| Suspension | Placebo3 | 26.15 ± 1.51 | |
| AMA1-C1/Alhydrogel Formulation | 26.41 ± 3.09 | ||
| 3 | Suspension | AMA1-C1/Alhydrogel, prior to Extraction | 29.15 ± 4.06 |
| AMA1-C1/Alhydrogel, Standard Extraction Method4 | -7.94 ± 1.65 | ||
| AMA1-C1/Alhydrogel, Standard Extraction Method with SDS5 | -17.7 ± 3.45 | ||
| AMA1-C1/Alhydrogel, Standard Extraction Method with CPC6 | 15.95 ± 1.97 | ||
| 3 | Supernatant of Vaccine Extractions | AMA1-C1/Alhydrogel supernatant, Standard Extraction Method4 | -7.99 ± 1.61 |
| AMA1-C1/Alhydrogel supernatant, Standard Extraction Method with SDS5 | -21.88 ± 1.75 | ||
| AMA1-C1 supernatant, Standard Extraction Method with CPC6 | 10.67 ± 8.39 | ||
1
Number of years vaccine formulations stored at 2-8°C;
2
Reference was prepared freshly
3
Placebo =1600μg/mL Alhydrogel in pH 7.0 saline;
4
Standard extraction solution = 0.6 M sodium citrate dihydrate /0.55 M sodium phosphate dibasic, pH 8.5
5
30 mM SDS (sodium dodecyl sulfate)
6
20 mM CPC (cetylpyridinium chloride)