Table 3.
Sandwich ELISA values for plasma cTnI and cMyBP-C levels
| Rat | Human | |||||
|---|---|---|---|---|---|---|
| Sham (n=7) | MI (n=11) | Fold of change | Normal (n=11) | MI (n=15) | Fold of change | |
| cTnI | 0.79±0.02 (ng/ml) | 7.0±0.5* (ng/ml) | 0.24±0.07 (ng/ml) | 30±9* (ng/ml) | ||
| 33 (fmoles/ml) | 292 (fmoles/ml) | 9 | 11 (fmoles/ml) | 1250 (fmoles/ml) | 114 | |
|
| ||||||
| cMyBP-C | 1.22±0.4 (ng/ml) | 109±4* (ng/ml) | 0.95±0.34 (ng/ml) | 227±50* (ng/ml) | ||
| 9 (fmoles/ml) | 775 (fmoles/ml) | 86 | 7 (fmoles/ml) | 1613 (fmoles/ml) | 230 | |
Values are expressed as mean ± SEM.
*
P< 0.001, control vs. MI. The mean values (ng/ml) were used to convert into fmoles/ml in order to demonstrate the number of molecules per ml. Data show that plasma levels of cMyBP-C in MI are significantly higher, compared to sham and controls. However, because ELISA antibodies only recognize the full-length and N′-fragments, cMyBP-C quantitation based on this method may not represent the exact amount of cMyBP-C present in the plasma samples. Femtomole, fmole (10−15 of a mole).