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Agreement between fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) for detecting RB1 loss in 33 glioblastomas (GBM). IHC and FISHshowed high concordance when FISH showed either retained copy numbers or homozygous deletions. The majority of tumors with hemizygous RB1 deletion by FISH retained RB1 expression by IHC.
