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. 2011 Jul 14;6(1):21–30. doi: 10.1038/ismej.2011.89

Figure 2.

Figure 2

PCR-based amplification of bacterial DNA extracted from historical CPR samples dating back to August 1961. Melting curve analysis (a) and agarose gel (b) showing the output of real-time PCR amplification of a 113-bp DNA fragment targeting the 16SDNA gene of Vibrio spp. from genomic DNA extracted from archived formalin-fixed CPR samples from the North Sea off the Rhine estuary dating back to August 1961.