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. 2011 Nov 25;13(1):44–51. doi: 10.1038/embor.2011.221

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Copyright © 2012, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

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Expression of SYCP3 leads to increased DNA double-strand breaks (DSBs), aneuploidy and hypersensitivity to DNA-damaging agents. (A) Immunofluorescence visualization of γH2AX foci (green) in RPE cells transfected with an empty vector (upper panel) and in RPE cells expressing SYCP3 (lower panel). Scale bar, 10 μm. (B) Percentage of cells containing more than three large γH2AX foci. A total of 100 cells were examined for each cell clone. (C) Microscopy images of interphase fluorescence in situ hybridization in SYCP3-expressing cells using probes for chromosomes 7 (Chr., orange) and 17 (green). Scale bar, 10 μm. (D) Percentage of cells containing one, three or four copies of chromosomes. A total of 500 cells were examined for each cell clone. In B and D, columns and bars represent the mean of three independent experiments and s.d., respectively. (E,F) Sensitivity to ionizing radiation (IR) and cisplatin. The representative result of three independent experiments is shown. The symbols and bars represent mean and s.d. of the triplicate dishes, respectively.