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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2012 Jan;71(1):54–65. doi: 10.1097/NEN.0b013e31823ede59

Figure 3.

Figure 3

Expression of JCV T antigen (Ag) and VP1 capsid protein in cortical neurons of PML patients. (A) Double immunofluorescence (IF) with an anti-T-Ag antibody (v-300, Alexa 568, red) and the neuronal marker anti-MAP-2 (M4403, Alexa 488, green) in an HIV-negative PML patient with malignant thymoma shows one cell in the gray-white junction (GWJ) expressing T Ag (box). Higher magnification views of cells in this box shown in the lower inset, and viewed separately with the green (middle inset) and red channel (upper inset) indicates that this neuron, recognizable by its cytoplasmic staining with MAP-2, expresses T Ag in its nucleus (MAP-2-positive/T Ag-positive cell, arrow). An adjacent uninfected neuron is marked by an arrowhead. (B) T Ag-expressing neurons in an HIV-positive PML patient using double IF with anti-T Ag (v-300, Alexa 568, red) and neuronal marker anti-NeuN (MAB377, Alexa 488, green). One NeuN-positive/T Ag-positive neuron in the box shown in the lower inset, and viewed separately with the green (middle inset) and red channel (upper inset) indicates that this neuron express T Ag in its nucleus (NeuN-positive/T Ag-positive cell, arrow). An adjacent uninfected neuron is marked by an arrowhead and an infected glial cell by an asterisk. (C) An HIV-negative PML patient with chronic lymphocytic leukemia has productively JCV-infected cells in grey matter (GM) shown by double IF with anti JCV VP1 (PAB597, Alexa 488, green) and anti-MAP-2 (ab5622, Alexa 568, red); one of them is a neuron expressing VP1 protein (VP1-positive/MAP-2-positive, arrow) seen in the lower inset, viewed separately with the green (upper inset) and red channel (middle inset). (D) A large neuron stained by double immunohistochemistry with anti-MAP-2 antibody (ab5392, VIP, purple) expresses JCV T Ag in its nucleus (v-300, brown) in an HIV-positive PML patient (arrow). An adjacent uninfected neuron is marked by an arrowhead. Scale bars: AC, 100 μm; D, 25 μm.