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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2012 Jan;71(1):54–65. doi: 10.1097/NEN.0b013e31823ede59

Figure 4.

Figure 4

An HIV-positive patient with PML lesions in the cerebellar WM has a restrictive JCV infection in the cerebral cortex with isolated neurons expressing T antigen (Ag) but not VP1. (A) Low-power view of the cortical and subcortical areas of the cerebrum showing no demyelination (H&E/Luxol fast blue). The boxed area in the grey matter (GM) is magnified in (B) in which immunohistochemistry (IHC) shows numerous cells with morphological characteristics of neurons expressing JCV T Ag (arrows, v-300, brown). Two of these cells in the boxed area of this panel are seen at higher power in the inset. (C) Double immunofluorescence staining against JCV T Ag (v-300, Alexa Fluor 568, red) and the neuronal marker MAP-2 (ab5392, Alexa Fluor 488, green) confirms that these cells are neurons. Three MAP-2-positive/T Ag-positive neurons in the box are shown in the lower inset (arrows), and viewed separately with the green (middle inset) and red channel. An adjacent uninfected neuron is marked by an arrowhead. (D) Double IHC with JCV anti-JCV T Ag (v-300, blue) and neuronal marker Neuron-specific beta III Tubulin (ab78078, brown) shows a large cortical neuron infected by JCV. Scale bars: A, 5 mm; B, 250 μm; D, 25 μm.