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Longitudinal sections of BLB explants after (A–C) 3, (D–F) 4, and (G–I) 6 months of implantation in vivo as an ACL replacement. (A, D, G) H&E staining for observation of general structure and collagen fibers; arrows show blood vessels found in tissue. (B, E, H) CD31 immunostaining for observation of blood vessels; (C, F, I) NCAM immunostaining for observation of nerves. NCAM, neural cell adhesion molecule; WGA, wheat germ agglutinin.
