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. 2011 Sep 26;18(1-2):198–207. doi: 10.1089/ten.tea.2011.0022

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 5. — Differentiation of CPCs into smooth muscle cells and endothelial cells in vitro. (A) Quantitative real-time RT-PCR analysis of smMHC and CD31 expression in CPCs. Expression in differentiation medium (black bars) was compared with expansion medium (white bars). (B) Costaining for smMHC (red), α-actinin (red), and nuclei (DAPI in blue). Scale bars in upper rows: 100 μm; in middle and lower rows: 25 μm. (C) Quantification of the number of SMC (smMHC^POS) in the culture. Data are presented as the percentage of smMHC^POS cells over the total number of cells (DAPI^POS nuclei). The bars in A and C represent means±SD. Asterisks denote significant difference compared with expansion medium (**p<0.01; ***p<0.001). smMHC, smooth muscle-specific myosin heavy chain.