Fig. 4.

Short-term retention of human hematopoietic cells in the murine peritoneum. Donor cells consisted of human bone marrow cells, 23 weeks of gestation, which were phenotyped by flow cytometry and 7.5 × 10⁷ cells transplanted i.p. into NSG mice. Eighteen hours later, cells were recovered from the peritoneum, counted and subjected to the same phenotypic analysis. Analysis of fresh bone marrow cells and representative results of 3 transplanted mice are shown. Results show live cell (PI⁻) events expressing human CD59 and lacking high-level murine CD45 and TER-119 expression. Additionally, the analysis of CD3, CD56 and CD19 expression was limited to gated events with a typical lymphoid forward and side light-scatter. Percentages reflect the number of events found in the associated region among all PI⁻ human cells. Percentages are derived from a single measurement of donor cells and the mean of measurements from 3 transplanted mice.