Table 1.

Primer Sequences Used for Quantitative Real-Time Polymerase Chain Reaction

Gene symbol	Accession ID	Forward primers (5′ to 3′)	Tm, calculated (°C)	Reverse primers (5′ to 3′)	Tm, calculated (°C)	Tannealing (°C)
PECAM1	NM_000442	GCAGTGGTTATCATCGGAGTG	59	TCGTTGTTGGAGTTCAGAAGTG	59	60
VEGFA	NM_001025366	CCACCACACCATCACCATC	59	GCGAATCCAATTCCAAGAGG	60	56
CXCL12	NM_199168	TTGTGAGAGATGAAAGGG	50	TACCGTATGCTATAAATGC	49	56
HIF1A	NM_001530	AGCCGAGGAAGAACTATGAAC	56	ATTTGATGGGTGAGGAATGGG	62	63
GAPDH	NM_002046	ACAGTCAGCCGCATCTTC	56	GCCCAATACGACCAAATCC	59	56
HPRT1	NM_000194	TGACACTGGCAAAACAATGCA	62	GGTCCTTTTCACCAGCAAGCT	61	60
RN18S1	NR_003286	GGCGCCCCCTCGATGCTCTTAG	71	GCTCGGGCCTGCTTTGAACACTCT	69	56
RPLP0	NM_001002	TGCACAATGGCAGCATCTAC	59	ATCCGTCTCCACAGACAAGG	59	60
B2M	NM_004048	TACTCCAAAGATTCAGGTTTACTC	55	TTCACACGGCAGGCATAC	58	60
PPIA	NM_021130	ATGGTCAACCCCACCGTGT	62	TCTGCTGTCTTTGGGACCTTGTC	64	60