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. 2011 Dec 27;5(12):e1461. doi: 10.1371/journal.pntd.0001461

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D'Archivio et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Schematic representation of pTvLUC vector constructs (A); TvPRAC5′UTR, upstream of the TvPRAC region and containing spliced leader acceptor site; Tubαβ, intergenic region between alpha and beta tubulin genes; NeoR, neomycin phosphotransferase gene; Tubβα, intergenic region between β and α tubulin genes. Representation of the ribosomal promoter region cloned into pTv-Luc and associated luminescence in Relative Light Units (RLU) detected in the supernatant 48 h after epimastigote transfection with pTvSrDNA-Luc and pTvLrDNA-Luc (B).