Table 1. Titers and sensitivity of antisera of immunized mice.
Immunogen/Coating antigena | Mouse | Titerb | IC50 (ng/mL)c |
DBAP-OVA / DBAP-BSA | 1 | 4000 | 395 |
2 | 8000 | 432 | |
3 | 16000 | 513 | |
DBAP-BSA / DBAP-OVA | 4 | 16000 | 1624 |
5 | 8000 | 1172 | |
6 | 8000 | 1335 | |
DBCP-OVA / DBCP-BSA | 7 | 16000 | >107 |
8 | 32000 | >107 | |
9 | 16000 | >107 | |
DBCP-BSA / DBCP-OVA | 10 | 16000 | >107 |
11 | 16000 | >107 | |
12 | 8000 | >107 |
aThe coating concentration was 1 µg/mL.
bThe last dilution of serum that gave an absorbance at 492 nm of >1.0 was judged as the titer.
cIC50 represents the mean of 3 wells replicates.