Table 2. Summary of the results of cell fusion and hybridoma selection.

Fusion parameters	No. of wells	Ratio (%)
seeded	352	-
fused	321	91.2
positivea	222	69.2
competitiveb	3	0.935
no. of cloned hybridomasc	1	-

^aWells with antibodies that recognized 1 µg/mL of DBAP-BSA conjugate by inELISA (absorbance >1.0).

^bWells with antibodies that recognized free DBP (inhibition >50% by 5 µg/mL DBP). IcELISA was carried out with 1 µg/mL DBAP-BSA, and culture supernatants giving absorbances out of range were diluted until absorbance about 1.0.

^cHybridomas secreting antibodies with the lowest IC₅₀ for DBP were stabilized and cloned.