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. 2011 Dec 27;6(12):e29196. doi: 10.1371/journal.pone.0029196

Table 4. Absorbance of anti-DBP antibody bound to hapten-coated polystyrene wells.

Conjugation temperatures
EDC (%) 4°C 25°C 37°C
0.1 0.272±0.0213 0.573±0.0415 1.084±0.0351
0.2 0.386±0.0252 0.686±0.0253 1.478±0.0090
0.4 0.451±0.0416 1.011±0.0308 1.526±0.0283
0.8 0.462±0.0384 1.034±0.0552 1.520±0.0441

Absorbance at 492 nm under different concentrations of EDC and conjugation temperatures. Each data represents the mean of ±SD of three replicates (three plates on the same day).