Table 4. Absorbance of anti-DBP antibody bound to hapten-coated polystyrene wells.
Conjugation temperatures | |||
EDC (%) | 4°C | 25°C | 37°C |
0.1 | 0.272±0.0213 | 0.573±0.0415 | 1.084±0.0351 |
0.2 | 0.386±0.0252 | 0.686±0.0253 | 1.478±0.0090 |
0.4 | 0.451±0.0416 | 1.011±0.0308 | 1.526±0.0283 |
0.8 | 0.462±0.0384 | 1.034±0.0552 | 1.520±0.0441 |
Absorbance at 492 nm under different concentrations of EDC and conjugation temperatures. Each data represents the mean of ±SD of three replicates (three plates on the same day).