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. 2011 Dec 27;6(12):e29166. doi: 10.1371/journal.pone.0029166

Figure 1. RIP-Cre Dicer1Δ/Δ mice develop diabetes during the first 25 weeks of age.

Figure 1

A. PCR analysis of tail genomic DNA showing the genotypes of the mice used in the experiments, lane 1 RIP-Cre Dicer1Δ/Δ and lane 2 their control littermates RIP-Cre Dicer1Δ /wt B. PCR analysis of genomic DNA from sorted β-cells demonstrating deletion of both alleles of Dicer1 gene in RIP-Cre Dicer1Δ/Δ and deletion of only one allele in control littermates RIP-Cre Dicer1Δ /wt mice. Numbers below lanes represent the two PCR reactions (one for each allele) for each mouse strain. C. Cumulative diabetes incidence in RIP-Cre Dicer1Δ/Δ mice. Glucose was measured every week from the age of 3 weeks using a glucometer. Control littermates never develop diabetes or hyperglycaemia and are therefore not shown in the figure. D. Plasma blood glucose levels (mmol/l) measured in 4, 7 and 13 weeks old RIP-Cre Dicer1Δ/Δ mice and littermate controls as indicated. The number of animals within the different groups is indicated below. Data are presented as mean±sd. ***P<0.001.