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. 2011 Dec 27;6(12):e29436. doi: 10.1371/journal.pone.0029436

Figure 8. Effects by gp120 on oxidative stress and apoptosis in Caco-2 cell monolayers.

Figure 8

gp120 (0.8 nM) was added to apical or basolateral side of Caco-2 cell monolayers and GSH/GSSG ratio (A) and caspase-3 activation (B) were evaluated after 1, 24 and 48 hours. *p<0,05 vs control; #p<0,05 vs apical gp120 stimulation at the same time. After gp120 stimulation cells were collected and western blots were performed with anti-gp120 polyclonal antibody (upper panel) and anti-caspase-3 monoclonal antibody (middle panel). Normalization of western blot was performed with GAPDH in all experiments (lower panel).