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. 2011 Dec 27;6(12):e29376. doi: 10.1371/journal.pone.0029376

Figure 5. miRNA inhibition can de-repress the utrophin 3′-UTR and upregulate translation.

Figure 5

C2C12 cells were transfected with 5′Luc3′, pRL-TK and different antisense miRNA inhibitors or a scrambled control inhibitor, at a range of concentrations. Luciferase assays were performed 24 hours post-transfection. Firefly/Renilla ratios in the presence of miRNA inhibitors were normalized to ratios in the presence of a scrambled inhibitor. Inhibitors of let-7, miR-150, miR-196b and miR-206 increased normalized luciferase activity, whereas inhibitors of miR-133b and miR-296-5p did not produce any significant upregulation. Bars represent mean ± standard error from three independent experiments. * Significantly different from scrambled inhibitor by two-way ANOVA followed by Bonferroni posttests, p<0.05.