AKBA inhibits ascites and distant organ metastasis in an orthotopic CRC murine model. (A) AKBA inhibited the development of ascites in CRC. The number of animals with ascites was counted 40 days after tumor cell implantation, and the percentages of animals with ascites were plotted. V, vehicle; L, low (AKBA 50 mg/kg); M, medium (100 mg/kg); and H, high (200 mg/kg). (B) AKBA inhibited metastasis to the lungs, liver, and spleen. Mice were killed and their abdomens were surgically opened; the number of metastatic foci in each organ was counted. Values are the mean ± standard deviation, n = 6. V, vehicle; L, low (AKBA 50 mg/kg); M, medium (100 mg/kg); and H, high (200 mg/kg).