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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Stroke. 2011 Oct 6;43(1):199–204. doi: 10.1161/STROKEAHA.111.626911

Figure 3.

Figure 3

Treatment with two SPK inhibitors abolished the protective effects of isoflurane preconditioning. A specific SPK inhibitor (SKI-II, A and B) and an SPK2 isoform-selective inhibitor (ABC294640, C and D) were used to verify the role of SPK2 in cerebral preconditioning. Mice were treated with either inhibitor at 100 mg/kg or vehicle (PEG400) by oral gavage at 15 min before preconditioning (IsoPC) and allowed to recover for 24 hours before a 90 min-MCAo. Neurological scores were evaluated at 24 hours after reperfusion (B and D). Data are mean±SD (n=8). *** indicates p<0.001 when compared to naïve mice. NS indicates not statistically significant.