Table 1.
Physical parameters of biarsenical dyes and optimized tetracysteine binding sequences
| Pro-fluorophore | Abs./Em. (nm) | Peptide ligand | Kapp (μM) | QY | Extinction coefficient | pKa | Dithiol resistance | Ref. |
|---|---|---|---|---|---|---|---|---|
| FLAsH-EDT2 | 508/528 | FLNCCPGCCMEP | 0.48±0.16 | 0.85 | 70,000 M-1cm-1 | 5.5 | 5.6±0.3mM BAL | [8, 71, 86] |
| HRWCCPGCCKTF | 0.65 | 2.3±0.1mM BAL | [8, 86] | |||||
| CCPGCC | 0.7±0.1mM BAL | [8, 86] | ||||||
| FLNCCPSQPTYPGDDAPVED LIRFYDNLQQYLNVCCMEP | 5.8±0.7 | [71, 72] | ||||||
| FLNCCEWTWDDATKTWTWT CCMEP | 0.62±0.12 | [71, 72] | ||||||
| ReAsH-EDT2 | 593/608 | FLNCCPGCCMEP | 0.37±0.20 | 0.48 | 69,000 M-1cm-1 | 4.7 | 2.0±0.3mM BAL | [8, 71, 86] |
| HRWCCPGCCKTF | 0.40 | 0.8±0.1mM BAL | [8, 86] | |||||
| CCPGCC | 0.2±0.1mM BAL | [8, 86] | ||||||
| FLNCCPSQPTYPGDDAPVED LIRFYDNLQQYLNVCCMEP | 3.4±1.2 | [71, 72] | ||||||
| FLNCCEWTWDDATKTWTWT CCMEP | 0.38±0.17 | [71, 72] |