. Author manuscript; available in PMC: 2013 Aug 1.

Published in final edited form as: J Nutr Biochem. 2011 Sep 19;23(8):966–976. doi: 10.1016/j.jnutbio.2011.05.003

Table 3.

Levels of fatty acids in colonic mucosa as a % of total fatty acids

	% of total fatty acids^†^‡^§
	COX-1 null		COX-2 null		wild-type
fatty acid	oleate	fish oil	oleate	fish oil	oleate	fish oil
lauric (12:0)	2.08 ± 0.287	1.71 ± 0.208	2.04 ± 0.431	1.22 ± 0.241	2.03 ± 0.274	1.43 ± 0.202
myristic (14:0)	2.55 ± 0.278	3.07 ± 0.244^a	1.91 ± 0.266	2.00 ± 0.297^c	2.30 ± 0.250	2.32 ± 0.267
palmitic (16:0)	19.2 ± 1.69	20.9 ± 1.96	16.3 ± 1.68	18.3 ± 2.07	17.9 ± 1.98	18.8 ± 1.83
palmitoleic (16:1ω7)	5.16 ± 1.08	4.30 ± 0.661	2.91 ± 0.261	3.61 ± 0.679	4.86 ± 0.666	4.96 ± 0.686
stearic (18:0)	5.96 ± 0.870	8.08 ± 0.790	8.35 ± 1.29	10.9 ± 1.28	6.50 ± 0.818	9.94 ± 0.768^b
oleic(18:1ω9)	39.9 ± 2.59	35.2 ± 3.62^a	37.9 ± 3.98	25.7 ± 3.85^b ^c	41.8 ± 3.01	26.5 ± 3.09^b
linoleic (18:2ω6)	13.5 ± 0.766	14.9 ± 1.27	15.2 ± 0.782	17.4 ± 1.16	13.9 ± 0.681	15.7 ± 1.12
linolenic (18:3ω3)	0.434 ± 0.0925	0.425 ± 0.431	0.666 ± 0.174	0.638 ± 0.129	0.450 ± 0.0666	1.15 ± 0.332
eicosenoic (20:1ω9)	0.743 ± 0.169	1.45 ± 0.246^a ^b	0.761 ± 0.121	0.766 ± 0.303^c	0.917 ± 0.132	0.794 ± 0.0615
eicosatrienoic (20:3)	0.670 ± 0.106	0.419 ± 0.129^a	0.856 ± 0.170	0.622 ± 0.111	0.534 ± 0.0932	0.738 ± 0.0998
AA (20:4ω6)	7.28 ± 1.03	2.15 ± 0.808^a ^b	9.53 ± 1.62	5.27 ± 0.826^b ^c	6.53 ± 1.29	4.77 ± 0.675
EPA (20:5ω3)	0.422 ± 0.104	2.80 ± 0.997^b	0.487 ± 0.0855^a ^b	4.74 ± 0.764^b	0.319 ± 0.0917	5.21 ± 0.829^b
DHA (22:6ω3)	2.19 ± 0.282	4.58 ± 1.51^b	3.13 ± 0.635^a ^b	8.75 ± 1.92^b	1.90 ± 0.380	7.81 ± 1.19^b
% ω3 HUFA^□	24.8 ± 1.79	76.4 ± 2.38^b	26.1 ± 1.32^b	68.4 ± 1.53^b	25.1 ± 2.94	69.5 ± 1.82^b

AA/EPA	24.6 ± 3.80	0.802 ± 0.176	21.4 ± 2.00	1.13 ± 0.0607	28.1 ± 4.72	1.03 ± 0.129

^†

Total fatty acids were determined by saponification of mucosa, derivatization, and analysis by GC-MS

^‡

Values represent mean ± SEM

^§

Means that differ significantly within each row (2-way MANOVA with comparisons of one factor within the levels of the other factor, P<0.05), are denoted as follows:

significantly different than wild-type mice fed the same diet,

significantly different than oleate diet for mice with the same genotype and

Significantly different than COX-1 null mice fed the same diet. Raw data are presented here, but statistical analyses were performed on Box-Cox transformed data as described in the Materials and Methods section. MANOVA was not performed for AA/EPA ratios.

^□

The % of highly unsaturated fatty acids (≥20 carbons and ≥3 double bonds) that are ω3 (the ω3 HUFA score)