. Author manuscript; available in PMC: 2013 Jan 15.

Published in final edited form as: Dev Biol. 2011 Oct 29;361(2):326–337. doi: 10.1016/j.ydbio.2011.10.028

Table 1.

Phenotypes associated with Dscam alleles and conditional deletion

Genotype	Target cell type	Fasciculation	Cell Number	Spacing	Notes
Dscam^Del17/Del17	Pan-Retina	++++	++++	++++	First published Dscam mutant
Dscam^F/F	Pan-Retina	++++	++++	++++	Largely recapitulates frame shift alleles
Dscam^F/F Brn3b-Cre	Most RGCs	++++	++++ or N.D.**	++++	Minimal cofasciculation of DA and mRGCs
Dscam^F/F Opn4-Cre	mRGCs	++++	++++	++++	Opn4 Cre targets many types of RGCs; Minimal cofasciculation of DA and mRGCs
Dscam^F/F Thy1-Cre	Many RGCs and amacrine cells	++++	++++	++++	non-autonomous spacing defects in DA cells
Dscam^F/F Chx10-Cre	All or none in clonal columns	++++	++++	++++	Demonstrates requirement for homophilic signaling.
Dscam^F/F Pax6-Cre	All	++++	++++	++++	Peripheral/Ventral portion of retina
Dscam^F/F Pax6 Cre	Most amacrine cells	++++	N.D.	++++	Central/Dorsal portion of retina
Dscam^F/F TH Cre (Jax #008601)	DA cells	−	++ (variable)	++ (variable)	Some mice had wild type phenotype
Dscam^F/F Ptf1A Cre	GABAergic amacrine cells	++	−	++
Dscam^F/F SHH Cre	RGCs	+ (D.N.S.)	+ (D.N.S)	+ (D.N.S.)
Dscam^F/F ChAT Cre	Cholinergic amacrine cells	N.A.	− N.O.	N.A.	Cholinergic lamination phenotype not observed after targeting with Chat- Cre
Dscam^F/F TH-Cre B6.FVB(Cg)-Tg(Th- cre)FI172Gsat/Mmcd	DA cells	−	−	−	No Cre activity detected in DA cells
Dscam^F/F Esr-Cre E12 induction	Pan-Retina	++++	++++	++++	Largely recapitulates frame shift alleles
Dscam^F/F Esr-Cre P0 induction	Pan-Retina	− DA + mRGC	++ DA N.O. mRGC	++
Dscam^F/F Esr-Cre P5 or older induction	Pan-Retina	D.N.S.	N.O.	N.O.	Demonstrates early period of Dscam function

N.A. Not Applicable

N.D. Not Determined

N.O. Not Observed but not quantified

D.N.S. Data Not Shown