Table 3.
Represents the bactericidal activity inE. coliDH5α by agar well diffusion assay for free antibiotics and their respective Gnps conjugated form after different temperature and time stresses
| Agents | Zone of inhibition forE. coliDH5α strain in sq. cm |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ampicillin |
Streptomycin |
Kanamycin |
||||||||
| Free | Gnps conjugated | % Change | Free | Gnps conjugated | % Change | Free | Gnps conjugated | % Change | ||
| Incubated for 10 min at |
26 °C |
3.085 |
3.569 |
+15.688 |
2.189 |
2.453 |
+12.060 |
3.371 |
4.545 |
+34.826 |
| 50 °C |
2.806 |
6.141 |
+118.85 |
1.378 |
1.622 |
+17.707 |
2.063 |
3.663 |
+77.557 |
|
| 75 °C |
2.198 |
6.635 |
+201.87 |
1.116 |
1.411 |
+26.434 |
1.834 |
3.389 |
+84.787 |
|
| 90 °C |
2.107 |
6.707 |
+218.32 |
0.053 |
1.324 |
+2398.1 |
1.491 |
3.263 |
+118.85 |
|
| Storage at room temp. (25–28°C) for | 0 day |
3.085 |
3.569 |
+15.688 |
2.189 |
2.453 |
+12.060 |
3.371 |
4.545 |
+34.826 |
| 3 days |
2.929 |
3.142 |
+7.272 |
2.126 |
2.361 |
+11.054 |
2.283 |
4.199 |
+83.925 |
|
| 7 days |
2.646 |
2.823 |
+6.689 |
1.486 |
2.049 |
+37.887 |
1.562 |
4.024 |
+157.62 |
|
| 14 days |
2.561 |
2.593 |
+1.250 |
1.055 |
1.483 |
+40.569 |
1.501 |
3.879 |
+158.43 |
|
| 21 days |
2.540 |
1.941 |
−23.583 |
0.913 |
1.345 |
+47.317 |
1.338 |
3.645 |
+172.42 |
|
| 28 days | 1.965 | 1.209 | −38.473 | 0.547 | 0.945 | +72.761 | 1.239 | 3.459 | +179.18 | |
The concentrations of free as well as Gnps conjugated antibiotics are 50 μg/ml for ampicillin, 10 μg/ml for streptomycin and 50 μg/ml for kanamycin