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. 2012 Jan;135(1):40–50. doi: 10.1111/j.1365-2567.2011.03511.x

Figure 2.

Figure 2

Gas6 and ProS inhibit inflammatory cytokine expression in an autocrine manner. Mouse peritoneal macrophages were cultured in serum-free medium for 6 hr before treatment. (a) Effects of recombinant Gas6 and ProS on the LPS-induced inflammatory cytokine expression. The wild-type (WT) and TAM triple mutant (TAM−/−) macrophages were treated with lipopolysaccharide (LPS) after a 2-hr pre-incubation with the indicated doses of Gas6 and/or ProS for 4 hr. The relative mRNA levels of the cytokines were analysed using quantitative PCR. (b) Effects of the neutralizing antibodies against Gas6 and ProS on the expression of inflammatory cytokines in the basal condition. The WT and TAM−/− macrophages were incubated with the rabbit antibodies against Gas6 (Ab-Gas6) and/or ProS (Ab-ProS), and p38 (Ab-p38) for 5 hr. The relative mRNA levels of the cytokines were analysed using quantitative PCR. (c) Effects of the antibodies on the LPS-induced inflammatory cytokine expression. The WT and TAM−/− macrophages were treated with LPS alone or with LPS after a 3-hr pre-incubation with Ab-Gas6 and/or Ab-ProS for 4 hr. The relative mRNA levels of the cytokines were analysed using quantitative PCR. Data are the mean ± SEM of three experiments (n = 3 mice per experiment). *P < 0·05; **P < 0·01.