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. 2012 Jan;135(1):40–50. doi: 10.1111/j.1365-2567.2011.03511.x

Figure 3.

Figure 3

Toll-like receptor (TLR) ligand-mediated inhibition of Gas6 and ProS expression. (a–c), Time-dependent suppression of Gas6 and ProS expression by TLR ligands. The macrophages in the serum-free medium were treated with 100 ng/ml lipopolysaccharide (LPS) (a), 5 μg/ml poly(I:C) (b) and 200 nm CpG (c) for the indicated time. The relative mRNA levels of Gas6 and ProS were determined using quantitative PCR. (d) Suppression of Gas6 and ProS secretion by TLR ligands. The macrophages were treated with the TLR ligands individually or with the TLR ligands following 2-hr pre-incubation with respective TLR inhibitors: antagonists of TLR4 (Ant-TLR4) and TLR9 (Ant-TLR9), neutralizing antibodies against TLR3 (Ab-TLR3). The concentrations of Gas6 (left panel) and ProS (right panel) in the medium were measured using ELISA 24 hr following the treatments. Data are the mean ± SEM of three experiments (n = 4 mice per experiment). *P < 0·05; **P < 0·01.