Figure 3.

(A) Nitrite production in rat aortic smooth muscle cells. Results are means ±SEM of individual cultures from six rats. Treatment groups: control (C), LPS (L), aldosterone (A), mifepristone (M). Numbers beside drug abbreviations are concentrations in µg·mL⁻¹ (LPS) and µM (aldosterone or mifepristone). (B) Immunoblotting of five individual cultures run over two gels. From left to right, groups of three adjacent lanes show corresponding treatments for each culture (L = 1 µg·mL⁻¹ LPS; LA = mixture of L with 1 µM aldosterone; LAM = mixture of LA with 10 µM mifepristone). ‘P’ represents sample of mouse macrophage protein extract containing iNOS. (C) Treatment group means (±SEM) of iNOS/actin band intensity ratios from densitometry. *P < 0.05, **P < 0.01, comparing LPS alone with controls or with LPS/steroid mixtures, except where other group comparisons are indicated by bridging arrows.