Figure 7.

Fold changes in protein expression of ERK and phosphorylated ERK at Thr²⁰²/Tyr²⁰⁴ site (pERK) determined by elisa from the ventrolateral medulla obtained during phases I or II after rats had received a microinjection, bilaterally into the RVLM, of Mev (10 nmol), given alone or in addition to pretreatment with immunoneutralization of phosphorylated Shc (pShc^Y239/240 Ab; 1:20 or pShc^Y317 Ab; 1:20) or pharmacological blockade of Grb2/SOS (SH3b-p; 25 fmol), Ras (FTA; 40 pmol or Manumycin A; 40 pmol) or Raf-1 (GW5074; 40 pmol). Values are mean ± SEM of triplicate analyses on samples from four to six animals in each group. *P < 0.05 versus vehicle group and #P < 0.05 versus Mev group in the Scheffé's multiple-range analysis.