Figure 1.

The induction of memory-related genes in the hippocampus of aged Tg2576 mice is facilitated with sildenafil treatment. (A) Scheme showing times of injection, training and death. Administration of sildenafil induces c-fos (B), Arc (C) and pCREB (D) following fear conditioning training. Representative Western blot bands from hippocampal tissues of non-transgenic mice (Non-Tg), transgenic mice treated with saline (Tg2576 saline) or with sildenafil (Tg2576 sildenafil) are shown. The histograms represent the quantification of the immunoreactive bands in the Western blot. Data are expressed as mean percentage (±SEM) of the Non-Tg results (100%). n = 5–6 in each group. *P < 0.05, significantly different from Tg2576 saline mice (Kruskal–Wallis followed by Mann–Whitney post hoc test).