Figure 3.
No change is detected in the amyloid levels after chronic treatment with sildenafil in aged Tg2576 mice. (A) Levels of Aβ42 in the transgenic mice by elisa. Results are expressed as mean ± SEM n = 6–8 in each group. (B) Effects of chronic administration of sildenafil on full-length APP, and on APP C-terminal fragments, C99 and C83, in aged transgenic mice. The histogram shows the quantification of the immunochemically reactive bands in the Western blot of APP, C99 and C83 (representative bands are shown). Results are expressed as mean ± SEM n = 5–6 in each group. (C) Extracellular deposits stained with 6E10 antiserum were detected in both, saline- and sildenafil-treated Tg2576 mice. Amyloid deposits were absent in age-matched (Non-Tg) control mice. Representative hippocampal sections of Non-Tg, saline- (Tg2576 saline) and sildenafil- (Tg2576 sildenafil) treated Tg2576 mice are shown. Scale bar = 100 µm.