Skip to main content
. Author manuscript; available in PMC: 2011 Dec 28.
Published in final edited form as: Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Sep 12;84(3-1):031911. doi: 10.1103/PhysRevE.84.031911

FIG. 2.

FIG. 2

(Color online) Schematic outlining the SA approach. (a) A Si-Ni-Si multilayer reference structure, alkylated with mercapto-propyl-silane (MPS) to functionalize its surface with thiol groups, is subsequently reacted with the linker maleimido-C3-NTA to provide nitrilotriacetate end groups for ligation with Ni+2 ions, along with (b) histidines from the protein’s C-terminal His6 tag following incubation with VSD-OG. (c) Reconstitution of vectorially oriented VSD in a single phospholipid bilayer environment via exchange against POPC-OG in the presence of Bio-Beads. For aqueous environments, water is expected on both the proximal and distal (with respect to the substrate) sides of the bilayer. The VSD is shown as ribbon representation of its crystal structure [6].