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. 2011 Dec;164(7):1829–1844. doi: 10.1111/j.1476-5381.2011.01475.x

Figure 4.

Figure 4

Morphine treatment depletes thymocytes undergoing selection. Mice were treated with morphine (M), morphine and naltrexone (M + N), naltrexone (N) or placebo (P) and analysed 7 days later. Thymocytes were analysed for surface expression of CD4, CD8, CD44 and CD25 and intracellular expression of TCRβ. (A–C) Data shown represent one mouse from each group. (A) The percentages of thymocytes that were DN, DP, SP CD4+ and SP CD8+ cells are shown. (B) Thymocytes were gated on the DN population and analysed for CD44 and CD25 expression. The percentages of DN cells that were DN1, DN2, DN3E, DN3L and DN4 are shown. (C) Thymocytes were gated on DN3E, DN3L and DN4 thymocytes and analysed for intracellular TCRβ expression. The percentages of DN3E, DN3L and DN4 thymocytes that expressed TCRβ are shown. (D) TCRβ+ DN3E, TCRβ+ DN3L and TCRβ+ DN4 thymocytes were analysed for their cell cycle status. The percentages of cells that were in the S, G2 or M phase of the cell cycle are shown. *P < 0.05, **P < 0.01, significantly different from morphine-treated mice.