Figure 5.

Morphine treatment induces depletion of ISP CD8⁺ and TSP CD4⁺ thymocytes. Mice were treated with morphine (M), morphine and naltrexone (M + N), naltrexone (N) or placebo (P) and analysed 7 days later. Thymocytes were surface labelled with anti-CD4, anti-CD8, anti-CD24 and anti-TCRβ. Cells were gated on SP CD8⁺ (A) or SP CD4⁺ (B) thymocytes and analysed for CD24 and TCRβ expression. The percentages of SP CD8⁺ or SP CD4⁺ thymocytes in each gate are shown. Data shown represent one mouse from each group.