Figure 6.

Peripheral T-cells recover from morphine-induced depletion via homeostatic proliferation. Mice were treated with morphine or placebo for 7, 14 or 21 days. Untreated control mice are shown as a dashed line. (A) The absolute number of splenic (SP) and lymph node (LN) T-cells are shown. (B) The percentages of SP and LN CD4⁺ and CD8⁺ cells that were in the S, G2 or M phase of the cell cycle are shown. (C) Spleen and lymph node cells were analysed as described in the legend to Figure 3. The percentages of cells that were central memory or effector memory are shown. *P < 0.05, **P < 0.01.