Table 1.
Expression level of cell adhesion-relating molecules (VLA-4, 5, and syndecan-4) and induced cell adhesion or apoptosis by TNIIIA2 or Mg2+ administration in fresh AML cells from patients, peripheral blood cells from healthy adults, and hematopoietic tumor cell lines (the table was modified from [19]).
| Cells | Expression (%) | Adhesion | Apoptosis | ||||
|---|---|---|---|---|---|---|---|
| VLA-4 | VLA-5 | Syndecan-4 | +TNIIIA2 | +Mg2+ | +TNIIIA2 | +Mg2+ | |
| “Fresh AML cells” | |||||||
| Patient A | 98.2 | 88.8 | 48.8 | ++ | ++ | + | ++ |
| Patient B | 97.5 | 98.5 | 9.5 | − | + | − | ++ |
| “Peripheral blood cells” | |||||||
| Neutrophil | 6.7 | N.D. | 3.2 | − | − | − | − |
| Monocyte | 48.6 | N.D. | 2.0 | − | ++ | − | ++ |
| Lymphocyte | 40.5 | N.D. | 4.4 | − | ++ | − | + |
| “Cell lines” | |||||||
| B cell | |||||||
| Ramos | 96.5 | 3.2 | 92.1 | +++ | ++ | +++ | ++ |
| Raji | 94.3 | 65.3 | 2.3 | − | ++ | − | ++ |
| T cell | |||||||
| Jurkat | 92.8 | 96.6 | 42.4 | +++ | ++ | +++ | ++ |
| Erythroid | |||||||
| K562 | 9.3 | 97.2 | 66.7 | +++ | ++ | − | − |
| Myeloid | |||||||
| U937 | 98.1 | 98.2 | 87.5 | +++ | ++ | ++ | +++ |
| HL60 | 99.8 | 99.7 | 75.3 | +++ | ++ | ++ | + |
| THP1 | 68.5 | 20.7 | 30.1 | ++ | ++ | ++ | + |
| THP1 (+PMA) | 10.5 | 18.7 | 99.2 | ++ | ++ | − | − |
Expression of VLA-4, 5 and syndecan-4 was evaluated by flowcytometric analysis.