Figure 2.

Vasa localization and expression during hybrid dysgenesis. A. At 2–4 days, only low levels of Vasa is present in the germarium of dysgenic hybrids (2–4 Day, w¹ × Har), while Vasa is dispersed in the cytoplasm and concentrated in perinuclear foci (nuage) in reciprocal hybrid controls (2–4 Day, Har × w¹). B. At 21 days, by contrast, Vasa is present in the cytoplasm and nuage in both dysgenic hybrids and reciprocal control. Vasa also shows the expected accumulation at the posterior pole of stage 10 oocytes (right panels). C. Western blot for Vasa in control and dysgenic ovaries. At 2–4 and 21 days, reciprocal hybrids express a species of the expected 72 kD apparent MW. 2–4 day old dysgenic hybrids, by contrast, express low levels of this species and a prominent higher mobility band. At 21 days, however, the 72 kD band is restored. In panels A and B, the distribution of Vasa is shown on the left and a merge of Vasa (green), DNA (Blue) and Lamin-C (red) is on the right. Note that Lamin-C is prominent in the terminal filament cells that occupy the anterior tip of the germarium and in the stalk cells located between egg chambers. Scale bars = 20 μm.