Figure 3.

Microscopic analysis of U87 cells (a, b, c, d, e, f) and mouse splenocytes (g, h, i). (a) Untreated U87 cells; (b, c) U87 cells were treated with 100 μM/L jaceosidin for 24 h and 48 h, respectively, and morphological changes were assessed by phase contrast microscopy. (d) Untreated U87 cells; (e, f) U87 cells were treated with 100 μM/L jaceosidin for 24 h and 48 h, respectively. Dead cells stained red while viable cells stained green. (g) Untreated splenocytes; (h, i) splenocytes were treated with 100 μM/L jaceosidin for 24 h and 48 h, respectively. Dead cells stained blue. (j) Data are expressed as Mean ± SEM of three independent experiments. Columns not sharing the same superscript letter differ significantly (P < 0.05).