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Oligonucleotide sequences of the primers used in this study.
| Orientation and primer | Sequence (5'-3') |
|---|---|
| Forward | |
| GH45CBMPiEcoF | GAATTCGCTCAAGCTGAAACTGCATCCC |
| GH45PiEcoF | GAATTCGGAGACTTTGAGACAATCCCC |
| GH45CBM1EcoF | GAATTC GATGTCCCACTTTGGGGCCAA |
| Reverse | |
| CBMPiXbaR | TCTAGACCTGATGAAGTCGTTTCCTC |
| GH45CBMPiXbaR | TCTAGACCTTCGTCCGTACGAGCACA |
The restriction sites added to insert genes into pPICZαA are underlined.
