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Chromatogram illustrating elution of proteins in muscle tissue extract from a healthy chicken from a carboxymethyl cellulose column (3.4 × 30 cm) with a 0 M to 0.05 M NaCl gradient. The total volume of the gradient was 2,500 mL. Optical density of each fraction was determined at 280 nm. The fourth peak (CM-Fr 1) of the CM-cellulose chromatography column contained CA activity.
