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Western blotting after SDS-PAGE. A single band of CA-III from purified chicken CA-III (1), male WL-chicken hemolysate (2), and female WL-chicken hemolysate (3) is observed after staining by immunochemical method using anti-chicken CA-III serum. All bands had an approximate molecular weight of 28 kD. These data indicate that the antibody against chicken CA-III produced in the present study is specific to chicken CA-III.
