Voltage-gated sodium channel (VGSC) partial sequence consensus alignment for Anopheles punctulatus, An. farauti s.s., An. hinesorum, An. farauti 4, and An. koliensis. Consensus sequence for each member was created using VGSC sequence from multiple representatives of each species: An. punctulatus s.s. (AP) (GenBank accession nos. HQ173943–HQ173973), An. koliensis (AK) (HQ173974–HQ173993), An. farauti s.s. (AFss) (HQ173904–HQ173917), An. hinesorum (AH) (HQ173918–HQ173930), An. farauti 4 (An. farauti 4) (HQ173931–HQ173942). Consensus sequences were compared with An. longirostris (HQ173903). Coding region sequence is underlined. Asterisks denote positions of the super-kdr (M918T) and kdr (L1014F, L1014S) mutation sites. Ligase detection reaction (LDR) probe locations for species determination and kdr detection are denoted by gray (classification probe) and black (reporter probe) coloration.