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. 2012 Jan 1;86(1):140–151. doi: 10.4269/ajtmh.2012.11-0503

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Figure 3. — Detection of species-specific DNAs by the Anopheles species/kdr detection multiplex polymerase chain reaction (PCR)-ligase detection reaction (LDR)-florescent microsphere assay (FMA). Data represents a summary of 14 individual control experiments detecting AP, AK, AFs.s., AH, and AF4 as well as Plasmodium falciparum (Pf), P. vivax (Pv), P. malariae (Pm), P. ovale (Po), Wuchereria bancrofti (Wb) and human (HBS) genomic DNAs. DNAs known to contain the kdr mutations (TTT and TCA) and a sample that did not contain the kdr mutation (TTA) were also included to test kdr sensitive (kdr TTA) and kdr resistant (kdr TTT and kdr TCA) probes. Whereas genomic DNAs were added individually into the control reaction, LDR and FMA reactions included oligonucleotide probes representing all five Anopheles species as well as KDR- and both KDR+ probes. Numbers in parentheses next to species designations in the legend (30), (100), (68), (78), (12), (14), (28), (95) identify Luminex FlexMap microspheres (Luminex Corp., Austin, TX). “No DNAs” identifies a blank sample to which no genomic DNAs were added.