Figure 2. SOCE activity and STIM1 protein are nearly absent in adult heart.
(A) Prevalence of SOCE in adult and neonatal ventricular myocytes (n=78-89) from 3-5 independent experiments. (B) Representative Ca2+ traces of detectable SOCE recorded from neonatal and adult myocytes. (C) Quantitative real-time RT-PCR analysis of STIM1 mRNA levels and (D) protein levels by immunoblot in adult and neonatal heart. (E) Quantitative real-time RT-PCR analysis of STIM-L mRNA levels in neonatal and adult hearts.