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. 2011 Nov 3;8:152. doi: 10.1186/1742-2094-8-152

Figure 9.

Figure 9

PARP-1 abrogation does not impair microglial phagocytosis of Aβ. A, Phase contrast and fluorescence images show the same field of microglia cultures. FAM-labeled Aβ (5 μM, green) is internalized by both wt and PARP-1-/- microglia, with or without a PARP-1 inhibitor (PJ34, 400 nM). B, Quantification of microglial FAM-Aβ phagocytosis activity, assessed after a 24 h exposure (n = 3). C, Orthogonal confocal views of microglia (stained with Iba-1, red) and FAM-Aβ (green) after 24 h incubation. The PARP-1-/- microglia do not assume a reactive morphology, but nevertheless engulfed the FAM-Aβ (yellow merge).