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. 2011 Dec 28;6(12):e29065. doi: 10.1371/journal.pone.0029065

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Michaloski et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A labeled double-stranded oligonucleotide corresponding to motif MV12 was incubated with nuclear extracts from vomeronasal epithelium (A), olfactory epithelium (B), and brain (br.), liver (li.) or lung (lu.) (C). The Olf-1 binding site (Olf-1s) was used as a positive control. MV12 forms complexes with vomeronasal (A) and olfactory epithelia nuclear proteins (B). These complexes are competed by the addition of a 100-fold molar excess (competitor) of unlabeled MV12 oligonucleotide, but not by the addition of a 100-fold molar excess of unlabeled Olf-1s oligonucleotide (shown here only for the olfactory epithelium nuclear proteins, in B). The Olf-1s/olfactory epithelium protein complex is competed by the addition of a 100-fold molar excess of unlabeled Olf-1s oligonucleotide, but not by the addition of a 100-fold molar excess of unlabeled MV12 oligonucleotide (B). Complexes are indicated by arrows. The positions of the free probes are indicated by filled circles.